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RECOMBINANT SPIDER SILK AS BIOMATERIAL IN MEDICAL APPLICATION

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RECOMBINANT SPIDER SILK AS BIOMATERIAL IN MEDICAL APPLICATION
Presenter: Stefan Langer
Co-Authors: Zeplin PH, Mougin N; Jordan MC; Berninger AK; van Gelder P; Leimer A; Langer S; Scheibel T
Leipzig University Hospital

Silicone has become an indispensible medical product used in all fields of medicine. With advanced implant placement times, long term biocompatibility of products has significantly gained in importance. Spider silk is a novel material with high scientific potential in biomaterial research as it combines biocompatibility, mechanic stability and versatility. Biologically engineered recombinant spider silk enable the production of silicon surface covering membranes. Material based analysis, in vitro study and a long term animal model were conducted to examine whether biocompatibility of silicon surfaces alter following coverage with recombinant spider silk. The material based analysis was conducted to assess the hydrophilicity and roughness of modified silicone surfaces. During the in vitro study, the effect of covered versus uncovered silicone surfaces on proliferation and cytokine expression of representative primary cells was analyzed. To test biocompatibility a long term in vivo rat model was conducted for a period of 12 months (n=80 rats). Rats either received covered or uncovered implants, placed in the subcutaneous layer. After three, six and twelve months periprosthetic tissue was extracted and received histologic, immunocytochemic and molecular biologic review. The coverage with recombinant spider silk led to significantly increased hydrophilicity of silicone surfaces. Both in vitro and in vivo models were able to show that spider silk coverage reduces the proliferation of fibroblasts as well as the expression of pro-inflammatory and pro-fibrotic cytokines. Moreover, by impacting collagen synthesis, spider silk influences the synthesis of matrix metalloproteinases and their inhibitors on the construction and modification of the extracellular matrix. The in vivo rat model showed that recombinant spider silk used as coverage for silicone surfaces leads to reduced foreign body associated reactions. Moreover spider silk offers significantly increased biocompatibility. The biotechnological controllability and modification of protein composition are of special interest in procedures where long term load lead


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